Bigger Capabilities.
Our Breez™ bioreactor is the only mL-scale platform that offers a fully automated, continuous True Perfusion™ culture. So, you can do everything you’re doing with a stirred-tank reactor and then some – because you’ll get better control, the ability to easily run multiple experiments at once, and cost savings with every use.
The 2mL bioreactor with True Perfusion designed for rapid bioprocessing and cell therapy development.
4 pod system with base station, bottle rack, and computer with operating software
Erbi Breez: Product Specifications
Reactor Working Volume
2mL
Number of Inputs
4
Flow Rate Range
0.1 – 10 VVD
Pump Accuracy, Resolution
+/- 2%, 600 nL
Minimum Sample Volume
50 uL
Measurement Range
5.5 – 8.5
Measurement & Control Accuracy @ PH 7
+/-0.10
Range
0-200% air sat
Measurement & Control Accuracy
+/- 10%
KLA
up to 40/hr
Measurement Accuracy
+/- 0.25 C
Control Range
Ambient – 50C
Control Resolution
0.03 C
Linear Range (CHO)
0-50M cells/mL
Usable Range
0-150M cells/mL
Auto Cell Bleed
+/- 10% setpoint (typical)
- Liquid Flow and Volume
-
Reactor Working Volume
2mL
Number of Inputs
4
Flow Rate Range
0.1 – 10 VVD
Pump Accuracy, Resolution
+/- 2%, 600 nL
Minimum Sample Volume
50 uL
- pH Measure and Control
-
Measurement Range
5.5 – 8.5
Measurement & Control Accuracy @ PH 7
+/-0.10
- Dissolved Oxygen
-
Range
0-200% air sat
Measurement & Control Accuracy
+/- 10%
KLA
up to 40/hr
- Temperature
-
Measurement Accuracy
+/- 0.25 C
Control Range
Ambient – 50C
Control Resolution
0.03 C
- Optical Density
-
Linear Range (CHO)
0-50M cells/mL
Usable Range
0-150M cells/mL
Auto Cell Bleed
+/- 10% setpoint (typical)
Product Resources
Application Note: Using the Breez for T Cell Culture
Donor T cells were purchased pre-enriched using negative selection beads from a third-party vendor. AIM-V media and Dynabeads CD3/CD28 were purchased from Thermofisher…
Application Note: High Density Perfusion Culture: Stirred Tank Equivalence with the Breez
The KTH Stockholm (AdBIOPRO) group ran a series of perfusion experiments using Breez to mimic their existing stirred bioreactor and ATF System model.
POSTER: Cell Therapy Process Development with a 2 mL Perfusion Bioreactor
Download the poster presented at ECI 2022
POSTER: High Throughput Process Intensification with a 2ml Continuous Perfusion Bioreactor
Download the poster presented at ESACT 2022
PODCAST: Answering the call for small scale tools that are fit for purpose
Erbi Biosystem’s Breez bioreactor system is featured in Cell Culture Dish podcast. In this podcast, 2 industry veterans, Tom Ransohoff and Bert Frohlich discuss the challenges moving programs to continuous bioprocessing, as well as growing pressures on time, space and resources. They offer how the Erbi Breez system is able to respond to the demands for continuous bioprocessing PD tools and is compact and easy to use therefore addressing need for perfusion systems that consumes less resources.
WEBINAR: “Breez”ing the Way into Process Development with Perfusion Technology
Erbi Biosystem’s Breez Bioreactor System was featured in BPI’s Ask the Expert webinar. Dr. Kevin Lee provides an overview of how the Erbi Breez 2 mL Bioreactor System enables better process control and reviews a series of case studies from collaborators highlighting the capabilities of the system.
Open the box and run your first experiment in the same day
WE NAMED IT BREEZ, BECAUSE USING IT IS.
A fully integrated Erbi Breez™ microbioreactor is simple to set up and easy to run sophisticated feeding strategies, cell concentration, and media exchanges. Our single-use design, automated processes, sophisticated software controls means that you can run more experiments with better control and data.
- Able to support high cell densities – 250 M/mL and beyond
- Supports multiple cell types including CHO, T-Cells, yeasts, bacteria, and more
- Quick and easy setup gets you up and running fast
- 2 mL working volume, 50 μL sample size
- Integrated cell retention filter for continuous perfusion, cell concentration/washing and media exchange
- 4 liquid and 3 gas inputs per reactor
- kLa up to 40/hr
- Powerful & easy to use software gives you complete control and unmatched data
Erbi Breez Microbioreactor vs. Stirred-Tank Bioreactors
Conventional stirred tank bioreactors are, well, conventional. Erbi Biosystems took each function and found improved ways to perform it, but in a smaller, more efficient package.
Stirred-Tank
Erbi Breez
Filtration Collection
Cell retention for perfusion is accomplished with an add-on filter system.
The Erbi Breez handles this function with an embedded PES filter and integrated pump that pulls filtrate through and retains the cells in the bioreactor. The filtrate is collected in a bottle that is part of the consumable, which also includes a 1.5 mL sample tube of expended media.
Automatic Cell Bleed Control
Cell bleeds are usually conducted manually after calculating the required dilution or setting a constant bleed rate.
The Erbi Breez performs cell bleeds automatically in response to measured optical density in order to hold a constant cell density.
Volume Control
The working volume is maintained by balancing the inflow and outflow pumps and weighing the fluid sources, outputs, and reactor tank. This is a lot to set up, manage and maintain.
The Erbi Breez does much of this work for you automatically. It can maintain a constant working volume using its reconfigurable mixing membranes and fixed-chamber volumes. Its pumps are factory calibrated, so flow rates are reproducible across different devices. By configuring the mixing membranes so that two chambers are completely full and one chamber is completely empty, opening an output valve releases any excess liquid which restores the 2 mL working volume.
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