Imagine having more control, increased insights, and all the performance of benchtop bioreactors – with fewer people and less space requirements. With an Erbi Breez microbioreactor, it’s now possible.
Erbi Breez bioreactor is the only mL-scale platform that offers a fully automated, continuous True Perfusion™ culture. So, you can do everything you’re doing with a traditional bioreactor and more because you’ll get better control, the ability to easily run multiple experiments at once, and get more data. With the same number of people, the Erbi Breez system delivers 7-9 times more process development throughput than a 2L reactor, for less cost.
Imagine having more control, increased insight and all the performance of a 1L or 3L bioreactor with fewer people, less space requirements and lower OPEX/CAPEX requirements. With the Erbi Breez, it’s possible.
Need to do more development with limited and expensive materials, especially with Autologous Cell Therapy Process Development?
Each POD operates independently for temperature, media flow rate, pH, DO, CO2 and cell density control and up to four (4) PODs can run simultaneously on each Basestation. Each POD has its own internal memory storage and can run for 2 weeks before needing to reconnect to a computer to download stored data.
Self-contained, single-use consumable is functionally closed, gamma sterilized and ready to use. The Consumable includes the bottle rack and microbioreactor. The 2mL, perfusion microbioreactor integrates with pumps, mixing, cell retention and pH, DO and OD sensing.
Easy-to-use interface makes it easy to set up the experiment, monitor and control the PODs and microbioreactor consumable
The Hub includes the Basestation and CO2 Sensor Box. The Basestation holds the PODs and distributes electrical and pneumatic lines to each POD. The CO2 Sensor Box handles CO2 sensing for pH calibration and online CO2 headspace control for microbioreactor and bottles.
This kit includes everything necessary to characterize media to be used with the Breez system. The media used must be characterized to determine its relation between dissolved CO2 and pH. The pH sensors inside the cassettes can then be calibrated by adjusting the percentage of CO2 gas delivered to media, without adding or removing any liquid from the chamber during the run.
Media prep, system set up, calibration, and sterility checks
63 hours
9 hours
Experiment take down, cleaning, and storage
27 hours
1 hour
Number of people needed
3 people
1 person
~ 3 week media requirement
480L
480mL
Total person-hours per 12 reactors
~90 hours
~10 hours
We understand that there are lots of steps needed to set up and take down conventional bioreactors. We aim to save the customers time with a single use, fully disposable fluid path. All required bottles, pumps, filters, and online sensors are integrated into the consumable. This means no extra time spent on reactor assembly, sterilization, tear down, and clean up. Once your experiment is complete, dispose of the consumable and you are ready to start your next run. The small milliliter working volume also significantly reduces time spent on media and inoculation preparation as well as in process media exchanges.
An Erbi Breez system running 4 bioreactors requires 70% less bench space than the average 1L benchtop bioreactor
“We will continue to use the Erbi Breez system in the future as a platform to optimize media formulations with model-based optimization strategies. The availability of a fully continuous perfusion system with good automation and control of important process parameters such as pH, DO and cell density, will reduce our use of labor intensive and costly stirred-tank bioreactors operated in perfusion mode.”
Hubert Schwarz, Ph.D. Student
KTH Royal Institute of Technology
High performance cell culture for therapy development & personalized manufacture
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