As companies are expanding into development of new biotech products there are mounting pressures to be faster and managing costs becomes a challenge. Erbi Breez has been developed to allow customers the ability to culture cells without limitations. The Breez can serve as a scale-up or scale-down model for process intensification and continuous processes that require True PerfusionTM while providing high gas transfer, O2 delivery, and CO2 stripping with low mechanical stress. Having a closed loop control over pH, DO, temperature, and cell density all at a 2 mL scale enables the Erbi Breez to provide a great model for optimization of all scale independent process parameters.
Speed: Save time with a pre-sterilized microbioreactor cassette and integrated fluid delivery system with automated setup & calibration. No assembly, sterilization, or tear down time.
Individualized automation: Breez bioreactors can operate independently or in parallel. They have an integrated filter to autonomously manage perfusion flow, enabling hands free flow control for applications such as N-1 Perfusion, media exchange, or cell concentration.
High performance: Reach cell densities in perfusion culture (>200M CHO cells/ml).
Scale-down model: Understand your large-scale processes with 1000-fold reduction in media and reagent costs.
Hands Free Reliability: Maintains steady state perfusion for over 30 days with automated and integrated cell density, pH, DO, and temperature control.
Demonstrated Performance with CHO
Example 1: Scale-Down
- 20 experiments in 3 months
- Met customer expectations for cell density and protein titer
- Generated data comparable to equivalent 10L glass bioreactor experiments
- Enabled customer to test cell responses to mixing and different media formulations
- Significantly less touch time than running equivalent stirred tank reactors
< 0.5 L media for 4 runs combined
4 replicates validates reproducibility
More processes, run by fewer people, with less interaction and variability
Example 2: Convert Fed-Batch Process to Perfusion Process Capable of:
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- Stable operation for > 20 days
- Significant improvement in volumetric productivity
- Acceptable media usage ( ≤ 2vvd)
- Compatible product quality
Example 3: Stirred Tank Equivalence (Cell Density and Mab Graphs)
- Matches performance to a 200 mL stirred tank with ATF
- High cell density and similar growth rate
- Expected metabolite profiles
- Equivalent protein titer and quality
Example 4: Media Optimization
- 18 experiments to optimize media mixture containing 4 subcomponents
- Quickly determine optimized media mixtures
- Simple CSPR experiments with automated cell density control
